4%PFA 配制由刀豆文库小编整理，希望给你工作、学习、生活带来方便，猜你可能喜欢“合理配制”。
4％多聚甲醛配制方法
1)400 ml dd H20 + 1000 ul 1N NaOH
2)add 20 g paraformaldehyde
3)Heat to 65-70℃
4)Continue heating and stirring until paraformaldehyde is diolved
5)Let cool to room temperature
6)Add 50 ml of 10X PBS
7)pH to 7.3 with HCl
8)Sterile with filter,to 500ml.9)Store at 4℃, protected from light
