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Dendritic Spines in Depreion: What We Learned from Animal Models(字体：Times New Roman，三号，居中)
生理学专业(宋体，小三号，居中)
Qiao H1, Li MX1, Xu C1, Chen HB1, An SC1, Ma XM2.(字体：Times New Roman，小四号，居中)
Depreion, a severe psychiatric disorder, has been studied for decades, but the underlying mechanisms still remain largely unknown.Depreion is closely aociated with alterations in dendritic spine morphology and spine density.Therefore, understanding dendritic spines is vital for uncovering the mechanisms underlying depreion.Several chronic stre models, including chronic restraint stre(CRS), chronic unpredictable mild stre(CUMS), and chronic social defeat stre(CSDS), have been used to recapitulate depreion-like behaviors in rodents and study the underlying mechanisms.In comparison with CRS, CUMS overcomes the stre habituation and has been widely used to model depreion-like behaviors.CSDS is one of the most frequently used models for depreion, but it is limited to the study of male mice.Generally, chronic stre causes dendritic atrophy and spine lo in the neurons of the hippocampus and prefrontal cortex.Meanwhile, neurons of the amygdala and nucleus accumbens exhibit an increase in spine density.These alterations induced by chronic stre are often accompanied by depreion-like behaviors.However, the underlying mechanisms are poorly understood.This review summarizes our current understanding of the chronic stre-induced remodeling of dendritic spines in the hippocampus, prefrontal cortex, orbitofrontal cortex, amygdala, and nucleus accumbens and also discues the putative underlying mechanisms.(字体：Times New Roman，小四号，两端对齐)利用CRISPR/Cas9技术构建GLRX3基因敲除的HEK293细胞
系(宋体，三号，居中)
生物化学与分子生物学专业(宋体，小三号，居中)
代鑫 赵俊丽 杨沛艳 夏海滨*(宋体，小四号，居中)
为了构建GLRX3(Glutaredoxin 3)敲除的HEK293细胞系,根据该蛋白结构特性设计了靶向于GLRX3基因Exon5的4个sgRNA,并通过T7E1检测确认了所设计sgRNA的有效性。将携带Cas9及靶向hGLRX3-Exon5的sgRNA1的打靶载体转染HEK293,通过药物Puromycin和细胞克隆化筛选出稳定GLRX3基因敲除的HEK293细胞株,并通过测序确定GLRX3两个等位基因均发生移码突变。通过免疫印迹在蛋白表达水平确认了该基因的敲除。利用CRISPR/Cas9技术成功构建了GLRX3敲除的HEK293细胞株,其为探索GLRX3的功能和作用机制提供了有效的细胞模型。(宋体，小四号，两端对齐)
