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范文：Dear...., I am working on......(工作背景).And one of my projects focuses on........(工作内容)Isolating ROG cDNA seems to be a problem hard to get over for us（为什么要要）.One of my graduates has been working on it for a couple of months, but no progre（一定以导师和教授口气写，学生写人家可能不愿意给）.We are happy to find that your laboratory was the major source of the ROG cDNA in many publications.Therefore, I am writing to explore the poibility of getting ROG cDNA from your laboratory.If you were kindly offering us the DNA(pCI-ROG), it would be very helpful for us.Of course, the DNA will never be used for commercial purpose.We will acknowledge the DNA resource and cite your papers in all our publications based on the DNA.We are also willing to put you into the author list that’s neceary.Sure, We would like to sign any contract for the DNA transfer.[BR]One of my graduates is waiting for the DNA to set up his experiment, so your early response will be greatly appreciated.Sincerely yours, [BR]
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收到质粒后,一定要回去一封感谢信.并再次重申不会用于商业或临床目的.Dear Profeor Bru V:
I am working on immunology.And one of my projects focuses on the treatment of HBV.We want to construct a vector pHBV1.5 which your used in one of your paper(Proc.Natl.Acad.Sci.USA,vol88:1059-1063).Construct this vector seems to be a problem hard to get over for us.One of my graduates has been working on it for a couple of months, but no progre.We are happy to find that your laboratory was the major source of the pHBV1.5.Therefore, I am writing to explore the poibility of getting pHBV1.5 from your laboratory.If you were kindly offering us the vector, it would be very helpful for us.Of course, the plasmid will never be used for commercial purpose.We will acknowledge the plasmid resource and cite your papers in all our publications based on the plasmid.Sure, We would like to sign any contract for the plasmid transfer.One of my graduates is waiting for the vector to set up his experiment, so your early response will be greatly appreciated.结果给我寄过来了,他是用滤纸寄的,先用500ng质粒溶解在1ulTE里,然后滴在滤纸上用塑料袋封闭,用的时候同样使用50ulTE将质粒溶解在EP管中,短暂离心后上清即可用于细菌转化.他是用空寄的,所以不需要通过海关,一般半个月就到了.
